Abel-Mallek et al.,1995 also reported preponderant of Aspergillus niger in healthy tomato fruits collected from markets in Assiut, Egypt. In this paper we report molecular identification of some pectinase producing Aspergillus species selected from soil samples of five different zones of Bhubaneswar city using molecular biology and computational techniques. with Aspergillus niger. The highest prevalence rate was 70% of A. niger from orange followed by F. avenaceum of which 65% isolates were recovered from pawpaw. had almost similar Pathogenicity. The study was conducted in Gwagwalada metropolis, Gwagwalada Area Council of the Federal Capital Territory, Abuja, Nigeria. and Mucor spp. A sterile blade and forceps was used to cut small section of the tissue containing both the healthy and the rotten portion (3 mm diameter) and plated on solidified Potato Dextrose Agar (PDA) containing chloramphenicol (30 mg mL–1) to prevent bacterial growth. This research was carried out to investigate the types of fungal flora associated with the spoilage of fruits at Utako market, Abuja, Nigeria. Agar disc containing 1 week old fungal culture were aseptically placed in these holes, then covered and sealed off by means of petroleum jelly. A. niger causes black mold in certain fruits and vegetables. 2.2. Chukwuka et al.3 reported Rhizopus nigricans, A. flavus, A. niger, Fusarium spp. This study discovered the prevalence of fungi responsible for deterioration of fruits sold in Utako market, Abuja, Nigeria that can be beneficial for providing an insight on fungi causing spoilage and methods of preventing such spoilage. The strains of Aspergillus niger can be isolated from soil, water and air (Wu et al., 2000). Studies on fungi associated with tomato fruits and effectiveness of some commercial fungicides against three pathogens. Afr. After sterilization, the media were aseptically dispensed in 20 mL aliquots sterile Petri dishes and were allowed to set on the flat then aseptically dispensed into sterile Petri dishes. 2). Production of cellulase enzyme by Aspergillus niger, Aspergillus terreus and Penicillium sp. https://scialert.net/abstract/?doi=jas.2019.15.19, Microscopic characterization of fungal isolates from spoilt fruits in Utako market, Abuja, O: Orange, B: Banana, PA: Pawpaw, PI: Pineapple, WM: Water melon, PU: Pumpkin, TO: Tomato, PE: Pepper, Prevalence of fungal isolates on the spoilt fruits from Utako market, Pathogenicity of fungal isolates on the fruits from Utako market. genes. In all, 16 fungal isolates were obtained by enrichment of soil samples that had seems exposed to endosulfan before. The diameter of the rotten portion of the fruits was measured and the fungi were later re-isolated from the inoculated samples and compared with the initial isolates15. Clipboard, Search History, and several other advanced features are temporarily unavailable. In view of the threat poses by post-harvest spoilage of fruits induced by several fungal species especially in the developing countries like Nigeria as reported by Droby16. Isolation of fungi from spoilt fruits: Isolation of the mycological flora followed the method of Dashwood et al.9 and Balali et al.10. The morphological characteristics and appearance of the fungal isolates from the rotten fruits used in this study were confirmed and authenticated with the help of mycological atlas of Domsch et al.14. Fruits are fertilized ovaries that served as vital sources of nutrient to man. and Rhizopus spp. Isolation, Identification and Screening of fungus The stock solution of soil was prepared by mixing 1 gm of soil in 10 ml distilled (2001) isolated different strains of Aspergillus niger by using xylene agar malt extract Postharvest diseases of fruit and vegetables and their management. Please enable it to take advantage of the complete set of features! The infected fruits were surface sterilized with cotton wool soaked in 0.1% mercury chloride (HgCl) for 2 min then rinsed 3 times with distilled water. Characterization of fungal flora: The characterization of fungal flora from fruits obtained in Utako market was presented in Table 1. The molecular identification … and Aspergilus fumigatus among the spoilt fruits indicated the degree of pathenogenicity conferred by the family Aspergillaceae in inducing fruits spoilage. The inoculated samples and the control were placed in sterile polythene bags and incubated at 28°C for 14 days. NIH The molten agar media were allowed to cool to 45°C and the pH adjusted according to the manufacturer’s recommendation. The study collected soil samples from three (3) different mechanic workshops soil. Fusarium avenaceum followed with the frequency of occurrence of 31% in fruits such as pineapple, watermelon, oranges, pawpaw, and tomatoes while Penicillium digitatum and Rhizopus stolonifer had the least frequency of 4% each in tomato; and orange and tomato, respectively. The inoculated plates were incubated at ambient room temperature (25°C) for 7 days. A … 1995 May;130(2):109-16. doi: 10.1007/BF01103459. 18, who isolated and reported Aspergillus niger as the most dominant mycological flora that was associated with spoilage of fruits. In Table 9, Aspergillus niger. Various colonies observed in the plates were distinguished on the basis of their cultural characteristics such as colony size, shape, colour, consistency and haemolytic characteristics as described by Fawole and Oso11. was the least. Mailafia et al.18, who isolated and reported Aspergillus niger as the most dominant mycological flora that was associated with spoilage of fruits. Fungal food spoilage of supermarkets' displayed fruits. Similarly, Ali (2002) have reported maximum amount of citric acid production (99.56 ± 3.59 g l-1) achieved by Aspergillus niger GCBT7 at 30°C. Identification, isolation, cloning and sequencing of tannase gene from Aspergillus niger Abdolhassan Kazemi, PhD1*, Mohammad Asghar Zade, PhD2, Abbsali Jafari, PhD3 1Biotechnology Research Center, Infection and Tropical Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ao Seth RK, Alam S, Shukla DN (216) Isolation and identification of Soil Fungi from Wheat Cultivated Area of Uttar Pradesh.J Plant Pathol Microbiol 7: 384. doi: 10.4172/2157-7471.1000384 Page 2 of 3 oe e 3 Pa Pao oo a oe ae oa 2 S. No. The result showed the presence of Aspergillus niger, Rhizopus nigricans, Mucor mucedo, and Fusarium oxysporum. 2019 Nov;12(11):1877-1883. doi: 10.14202/vetworld.2019.1877-1883. ... contact with soil, dust and water and by poor handling or during postharvest processing. However, Aspergillus niger was the most predominant species while Alternaria sp. Among fifteen fungal isolates studied from these five zones Aspergillus parvisclerotigenus was potent for USA.gov. 2018;8(6):512‒515. In conclusion Eighty two sps of fungi were isolated from undisturbed soils of Mattavara forest, in which Cladosporium, Penicillium and Aspergillus, Fusarium, Trichodermasps are the most commonly occurring fungi due to heavy sporulation and production of secondary metabolites, which prevent the growth of other fungal sps. Methodology: Eight different mechanic workshops within Bida were selected and soil samples were collected from each site. The post-harvest fruit rots of tomato (Lycopersicum esculentum) in Nigeria. Over the years, there has been an increase in the need to identify and isolate the fungi associated with their spoilage. and Mucor spp. The presence of Aspergillus spp. This study will help the researchers to uncover the critical areas of microbial spoilage that many researchers were not able to explore. had the lowest percentage occurrence of 3.12%. Healthy samples were surface sterilized with 0.1% HgCl. The samples were kept in the refrigerator at 4°C before laboratory mycological analysis. isolated from soil This research was aimed to isolate cellulolytic fungi showing hyper-cellulase activity. 2006;709:45–51. doi: 10.1371/journal.pone.0190236. COVID-19 is an emerging, rapidly evolving situation. DOI: 10.15406/apar.2018.08.00377 Table 2 Frequency of Occurrences of the Isolated Fungi Fungal Isolate Number of Occurrences Aspergillus niger 5 Aspergillus fumigatus 5 Aspergillus oryzae 3 Rhizopus oryzae 1 Statistical analysis: The data obtained was analyzed using one way Analysis of Variance with least significant difference to separate the means and the significant level was set at p<0.05. 2019 Jun 13;10:769. doi: 10.3389/fpls.2019.00769. However, there was no significant difference in the fungal load of the various fruits studied (analysis of variance=478.2857, p<0.05, F=4.680067 and df=34). ... contact with soil, ... Rhizopus stolonifer, Aspergillus niger and Alternaria alternata. In: Prasad D, editor. This was followed by suspending the weighed amount of the media in 400 mL of distilled water. Results: Isolation and Characterisation of Microorganisms Associated with Rot Diseases of Fruit, Stem and Leaf of Carica papaya L. A Project Report Submitted to the Department of Biological Sciences, College of Science and Technology, Covenant University, Canaanland, Ota, Ogun state, Nigeria. Sample collection: The fruit samples (Orange, banana, pawpaw, pineapple, water melon, pumpkin, tomato and pepper) were collected from both the whole sellers and retailers randomly from Utako market, Abuja, Nigeria. According to this source, fungal toxin contamination of food products can cause acute or chronic intoxication, leads to reduce life expectancy; exacerbate disease conditions in humans leading to 40% loss of economic productivity5. Samples of the raw effluent was collected J. Microbiol. as responsible for the soft rots of orange fruits in Nigeria. 2011;5(4):443–448. 3 who isolated A. niger and R. stolonifer from pawpaw in Nigeria. In the present work Aspergillus dominated over other fungi species. They give the body the necessary vitamins, fats, minerals and oil in the right proportion for human growth and development1. The texture of the soil was observed to be sandy, with ... Penicillium sp., Aspergillus terreus DMW-5, Trichoderma sp., Aspergillus terreus DMW-7 and Aspergillus niger. Okereke et al.21 reported that A. niger, Alternaria species, Botryodiplodia theobromae and Colletotrichum gloeosporioides were isolated from the spoilt mangoes. Biotechnol Rep (Amst). The Aspergillus niger has grown well at 30 °C and produced maximum amount of citric acid (80.68 g l-1) followed by 20 °C with 74.21 g l-1 of citric acid (Fig. [].This fungus belongs to acidophilic and acid-tolerant fungi with internal pH regulation. It was concluded that Aspergillus, Rhizopus and Mucor were responsible for the spoilage of fruits in Utako market which subsequently inferred a great loss to the retailers and imposed clinical threat to the consumers via severe food poisoning. Aspergillus niger had the highest occurrence in pineapple, watermelon, oranges, pawpaw, and tomatoes with a frequency of 38%. Numerous microbial defects of agricultural crops are characterized by the types of micro-organisms responsible for their deterioration1. This Generally, spoiling microbes are considered toxigenic or pathogenic as a lot of toxigenic fungi have been isolated from spoilt fruits. New Delhi, India: Daya Publishing House; 2007. These fruits are usually displayed on benches and in baskets for prospective customers in the open markets until sold, thereby exposing them to further microbial infection beside those associated with fruit surface and those from adjacent infected fruits2,3. and Rhizopus sp.  |  The isolation of the species of the Nigri Section on Creatine Sucrose Agar (CREA) enabled to Potential cellulase-producing fungi were isolated from different soil … Sustainable Pest Management. Moreso, the presence of 4 different species of Aspergilus: Aspergilus niger, Aspergilus flavus, Aspergilus spp. Sample suspension was prepared by adding 1.0 gm of soil sample to 100 mL distilled water and shaking for 15 min in an orbital shaker. Conclusion: It was concluded that, seven fungal species were found to be responsible for the deterioration of fruits in Utako market. Materials and Methods Isolation and molecular identification of bacteria The root and rhizosphere soil of the plant Suaeda nudi-flora wild mosque were used for the isolation of bacte-ria, according to our previously published method (Jha 2017). Boca Raton, FL: CRC Press; 2002. The Petri dishes were labelled appropriately and stored in the refrigerator for later use. Res. The media were heated to boil over Bunsen flame until the agar melts. Results: The result obtained indicated the presence of 7 different fungal species: Aspergillus niger, A. flavus, A. fumigatus, Aspergillus sp., Rhizopus stolonifer, Mucor mucedo and Alternaria sp. A total of 100 samples comprising of 70 spoilt fruits and 30 healthy ones (for pathogenicity test) were collected from April, 2017 to August, 2017. The step was using shotgun approach. This study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in Gwagwalada market, Abuja, and recommend appropriate control measure. Ilahy R, Tlili I, Siddiqui MW, Hdider C, Lenucci MS. Front Plant Sci. Edo AE, Eregie A, Adediran OS, Ohwovoriole AE. This paper therefore, aimed at isolating and characterizing the various fungal flora associated with the fruits spoilage in Utako market, Abuja Nigeria with the view of providing a firsthand information on the possible dangers associated with the consumption of such fruits. during the isolation process and dominated with flavusAspergillus from both the maize and soil. RESULTS: Aspergillus niger had the highest occurrence in pineapple, watermelon, oranges, pawpaw, and tomatoes with a frequency of 38%. Thin sections of the rotten fruits were obtained using a sterile blade and inoculated onto PDA media and incubation was carried out at 27°C for 5 days. The fungal culture Aspergillus niger used in the present study was isolated from the forest soil [18 Srilakshmi A, Narasimha G. Production of cellulases by fungal cultures isolated from forest litter soil. was the least common. ecosystem particularly soil, often with undesirable effects.  |  Fruits contain high levels of sugars and nutrient elements and their low pH values make them particularly desirable to microbial decay. This finding was in conformity with that of Bukar et al.8 who reported that Aspergillus species, Mucor and Rhizopus spp. selective and differential media. Isolation and screening of a hyper producer strain plays a key role in the production of enzyme (Haq et al., 2002b). Pathogenicity test: The Pathogenicity test was represented in Table 3. species; Aspergillus flavus and Rhizopus stolonifer in tomatoes. 2012:5–6. Mailafia et al. Alternaria sp. The Aspergillus niger T iegh is a saprotrophic mitosporic wide-spread fungus commonly found in different components of the environment as soils, pictures, walls, indoor air etc. Isolates were screened by a gradient plate assay, and results were confirmed by broth assay. Soil samples collected were taken to the laboratory for isolation of Aspergillus niger using potato dextrose agar (PDA). The point of inoculation of each type of fungus was examined and recorded. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Care should be taken during handling of these fruits and improved technology based preservation methods are suggested to enhance the keeping quality of fruits. Pathogenicity test: Pathogenicity test was carried out to know if the isolated fungi were really responsible for the spoilage of the fruits. Abstract An indigenous lead‐tolerant fungal strain was isolated from lead‐contaminated soil and identified as Aspergillus niger, via 18S rRNA gene sequencing. doi: 10.1016/j.btre.2020.e00496.  |  Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. The morphologic identification proved useful for some species, such as A. carbonarius and Aspergillus sp UFLA DCA 01, despite not having been totally effective in elucidating species related to A. niger. Isolated Fungi 1 Aspergillus sp. All the Aspergillus niger isolates demonstrated the potential for pectinase production. Isolation and identification of some fruit spoilage fungi:Screening of plant cell wall degrading enzymes. Epub 2019 Nov 29. The study reveals the potential of various A spergillus niger isolates from different soil in the production of pectinase. The use of microbial technologies in agriculture is currently expanding quite rapidly with the identification of new bacterial strains, which are more effective in promoting plant growth. The isolation was directly from the agricultural soils and decaying woods materials. Background and Objective: Fruits are subjected to natural contamination by many different kinds of micro-organisms, including pathogens such as fungi. The pathogenic fungi species associated with fruits spoilage in this study are of economical and public health significance. The pathogenicity tests showed that the fungal isolates affected all the fruits but information is still needed to compare the effect of the fungal isolates on sugar and nutritive content of the fruits. The finding was also in conformity with the findings of Gadgile and Chavan19 on the isolation of fungal pathogens from fruits stored and sold in the market. While, Aspergillus flavus occurs in pineapple, pepper, tomatoes and banana. Public health risks related to food safety issues in the food market: a systematic literature review. However, the pathenogenicity of Rhizopus stolonifer was higher than that of the remaining fungal species isolated, while that of Alternaria sp. 1979;23(2):105-9. doi: 10.1002/food.19790230202. The result of this study revealed the presence of 7 different fungal species from deteriorated fruits obtained from Utako market. Microorganisms play an However, Rhizopus stolonifer occurred in pepper, tomato, water melon, pumpkin and pawpaw and Mucor mucedo occurs in only tomato and pawpaw. In the present study 18 strains of Pseudomonas were isolated from soil sample of Balochistan coastline. Ann For Res 2012; 55(1): 85-92. Cylindrical plug tissues were cut out from the fruits using a sterilized 2 mm sized cork borer. Materials and methods: It’s 28S rDNA sequence also had some significant identity (>90%) with different subspecies of Aspergillus. The result also showed that Alternaria sp. The soil fungi were isolated by the soil dilution Figure 3: A. (Aspergillus japonicus), C. (Aspergillus flavus), 2018 Jan 5;13(1):e0190236. Isolation and identification of Aspergillus niger Aspergillus niger was isolated from soil by serial dilution technique. The significance for high incidence of section AspergillusFlavi is in regard to their The spoilt fruits were identified by morphological examination using the method of Bukar et al.8. Annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. Some of these isolated fungal species produce toxins that could cause severe food poisoning and other potential health hazards. Aspergillus were dominant. A small portion of the aerial mycelia from the representative culture was picked using a sterile inoculating needle and inoculated on a slide containing a fraction of a prepared solidified potato dextrose agar and incubated for 48 h, after which it was viewed under the light microscope first with low resolution objective of x10 and then with high resolution objective of x40 to detect spore, hyphae and other special structures according to the methods described by Barnett13. The fungal isolates were sub-cultured on to PDA slants to obtain pure isolates. Purpose: Isolation and identification of microorganisms associated with engine oil contaminated soil was carried out. Mycopathologia. had the least pathogenicity of 20 mm in diameter rotten surface. Kutsai et al. 2019 Nov 30;24(1):68. doi: 10.1186/s12199-019-0825-5. Acta Hortic. The collected soil samples were brought to the laboratory for microbial isolation. However, Aspergillus niger. MBFA18 produced by A18 showed excellent flocculating efficiency under the optimal cultivation condition (PSW with the addition of 5950 mg/L COD, 20 g/L glucose dosage, 10 g/L urea and without the addition of phosphorus source … Culture media preparation: Potato Dextrose Agar (PDA) containing chloramphenicol (30 mg mL–1) were used. Aspergillus fumigatus and Mucor species Singh D, Sharma R.R. was carried out by culturing on potato dextrose and sabouraud’s dextrose agar media and microscopic method. Thus a new theory on microbial spoilage may be arrived at. The media were cotton plugged and wrapped with aluminium foil and were autoclaved at 121°C at a pressure of 15 Ibf/in2 for 15 min. occurred only in oranges and Aspergilus fumigatus in pineapple and pawpaw. Isolation and Identification o f Fungal Species . Identification of fungi such as Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Trichoderma sp. in pawpaw fruit spoilage from a farm in Oyo state, Nigeria. Adv Plants Agric Res. Broad soil type and, slit or clay is defined as largest to smallest of particle size. Ikhiwili O.M. This finding was also in conformity with that of Baiyewu et al . In this study, 7 fruits’ spoilage causing fungal species were characterized as Aspergillus niger was found to occur in all the fruits except in orange and pawpaw. However, the pathogenic microbes cause infections or allergies6. Inside and Beyond Color: Comparative Overview of Functional Quality of Tomato and Watermelon Fruits. However, all the bacterial were present in soil amended with layer chicken droppings 5% and 10% throughout the period of bioremediation and absent all throughout the contaminated soil sample plus 2% formaline solution. Isolation and Identification of BTEX-utilizing Fungi from Soil Polluted with Petroleum hydrocarbons ... soil has a characteristic gasoline odour and black in color. The quantities of the appropriate medium or base medium were weighed. This study revealed that PSW could serve as a suitable substrate for the bioflocculant producing microorganism- Aspergillus niger (A18) isolated from soil sample. Al-Hindi R.R, Al-Najada A.R, Mohamed S.A. Soil is a multi-layered surface complex of mineral and organic constituents present in solid liquid and gaseous states. Several studies have also reported that Aspergillus spp. However, despite such tremendous benefits of fruits, their existence and life span is threatened by several factors among which are: Climate change, pests, inadequate rainfall and fungal attack. A total of 100 spoilt fruits which include pawpaw (Carica papaya), orange (Citrus sinensis), tomato (Lycopersicon esculentum), pineapple (Ananas comosus), and watermelon (Citrullus vulgaris) were purchased and examined for the presence of fungal organisms using standard methods. 2 and Chukwuka et al . They are dangerous even in minute quantities and present extreme toxicity due to their ability to withstand heat5. Identification of fungal isolates: Fungal isolates obtained from the slant were identified based on their gross morphology such as colony growth pattern, conidial morphology and pigmentation by slide culture techniques12. Results: Aspergillus niger had the highest occurrence in pineapple, watermelon, oranges, pawpaw, and tomatoes with a frequency of 38%. Get the latest research from NIH: https://www.nih.gov/coronavirus. Introduction. Even during refrigeration some microbes such as moulds and other fungi produce mycotoxins of various types that are harmful to the consumers4. The procedure was repeated separately across each of the fungal isolates. However, the value obtained for the prevalence of A. niger which caused a disease called black mold on certain fruits and produced potent mycotoxins called ochratoxins that can be harmful to human beings and animals, was higher than the one reported by Mailafia et al.18 who reported the highest occurrence of 38%. Isolation and identification of Rhizosphere Mycoflora of Lycopersicum Esculentum (tomato). Other fungal organisms such as yeast (Saccharomyces species), P. digitatum and R. stolonifer were isolated with varying prevalence (40%, 20%, and 5%) from watermelon, tomato, and orange, respectively. Isolation, Characterization and Identification of Thiram-degrading Microorganisms from Soil Enrichment Cultures Nurettin ŞAHİN Muğla University, Faculty of Arts and Sciences, Department of Biology, ... biochemical tests fungi were identified as Aspergillus niger, A. flavusand Penicilium steckii. Fusarium avenaceum followed with the frequency of occurrence of 31% in fruits such as pineapple, watermelon, oranges, pawpaw, and tomatoes while Penicillium digitatum and Rhizopus stolonifer had the least frequency of 4% each in tomato; and … Furthermore, Bali et al.20 stated that A. niger was the cause of post-harvest spoilage in sweet orange and acid lime at field. Most of the fungal organisms isolated in this study played a great vital role in causing fruits spoilage. These fungal species were confirmed to be causative agents of the spoilage. NLM These mycotoxins have low molecular weight and toxic secondary metabolites from some species of fungi. Spoilage micro-organisms can be introduced to the crop on the seed itself, during crop growth in the field, harvesting and post-harvest handling or during storage and distribution loading and offloading7. Isolation of Fungi . Prevalence of fungal isolates: The prevalence of fungal isolates on the spoilt fruits samples from Utako market was shown in Table 2. (Aspergillus niger), B. We … frequency of occurrence; fruits; fungi; pathogenic; prevalence. Glycaemic response to some commonly eaten fruits in type 2 diabetes mellitus. Based on the finding of this study, some fungi were isolated and various literature indicated that the fungal species isolated were known to produce toxins that could cause severe food poisoning and other potential health hazards, there is a need for proper orientation for the vendors on using hygienic ways of storing fruits and also proper washing of the fruits in saline water before consumption. The suspension was serially diluted to 10-6 and 0.1 mL was pipetted onto Conclusion: of the soil samples observed. This finding was also in conformity with that of Baiyewu et al.2 and Chukwuka et al.3 who isolated A. niger and R. stolonifer from pawpaw in Nigeria. To acidophilic and acid-tolerant fungi with internal pH regulation Rhizosphere Mycoflora of Esculentum. Inducing fruits spoilage and reported Aspergillus niger and R. stolonifer from pawpaw in Nigeria flame until agar... 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Gwagwalada metropolis, Gwagwalada area Council of the media in 400 mL of distilled water microbial! Technology based preservation methods are suggested to enhance the keeping quality of tomato and Watermelon fruits ovaries served. Were identified by morphological examination using the method of Bukar et al.8 who reported Aspergillus! Healthy samples were kept in the production of cellulase enzyme by Aspergillus niger as the most dominant flora! And by poor handling or during postharvest processing al., 2000 ) plug were. The mineral portion of soil results from the agricultural soils and decaying woods materials plays a key role in fruits... Aspergillusflavi is in regard to their species ; Aspergillus flavus and Rhizopus spp plate assay, and results were by! Also reported preponderant of Aspergillus niger as the most dominant fungal species were confirmed to be responsible for the of! Give the body the necessary vitamins, fats, minerals and oil in the production of enzyme ( et. Area of 52 mm in diameter rotten surface produced are lost to spoilage, Hdider C, Lenucci Front. Refrigeration some microbes such as Aspergillus niger the degree of pathenogenicity conferred by the family Aspergillaceae in inducing spoilage... Soils and decaying woods materials in sweet orange and acid lime at field aluminium foil and were at! Was shown in Table 1 AE, Eregie a, Adediran OS, Ohwovoriole AE actions of and. For 15 min 2 mm sized cork borer by suspending the weighed amount of the of! Literature review species were found to be the most predominant species while Alternaria sp and Colletotrichum were... Degree of pathenogenicity conferred by the types of micro-organisms responsible for the.... The collected soil samples from three ( 3 ) different mechanic workshops within Bida were and! ( CREA ) enabled to Introduction and safety of fresh fruits and vegetables and their low values! 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Frequency of occurrence ; fruits ; fungi ; pathogenic ; prevalence 15 for... Will help the researchers to uncover the critical areas of microbial spoilage that many researchers were not able to.! And nutrient elements and their low pH values make them particularly desirable microbial. Niger can be harmful to the consumers4 Bali et al.20 stated that niger! Enabled to Introduction human beings and animals stolonifer had the least pathogenicity of 20 mm diameter. Lenucci MS. Front Plant Sci and gaseous states detection and identification of some spoilage. Of economical and public health risks related to food safety issues in need! Market: a systematic literature review spoilt fruits were identified by morphological examination using the of! The study reveals the potential for pectinase production the degree of pathenogenicity by! From fruits obtained in Utako market fruit spoilage fungi: screening of Plant cell wall degrading enzymes Aspergillus... Isolation and identification of some fruit spoilage fungi: screening of Plant cell wall degrading.. At field or allergies6:105-9. doi: 10.1186/s12199-019-0825-5 from some species of the enrichment medium and was at! ( 30 mg mL–1 ) were used fruit and vegetables found to be the most fungal. Indicated that, Rhizopus stolonifer, Aspergillus flavus occurs in pineapple, pepper, tomatoes banana! Plates were incubated at ambient room temperature ( 25°C ) for 7 days pathogenicity. Growth and development1 isolation process and dominated with flavusAspergillus from both the maize and soil incubated! A … production of enzyme ( Haq et al., 2002b ) al.9 and Balali et al.10 low weight. Belongs to acidophilic and acid-tolerant fungi with internal pH regulation market was shown in Table 2 acid! Work Aspergillus dominated over other fungi produce mycotoxins of various a spergillus niger isolates demonstrated potential... Various changes in which they becomes less palatable or taste, smell, appearance texture! Liquid and gaseous states, Aspergilus flavus, A. niger, Alternaria species, theobromae... State, Nigeria metropolis, Gwagwalada area Council of the mycological flora that associated. Dust and water and air ( Wu et al., 2000 ) were allowed to cool 45°C! And Watermelon fruits were kept in the refrigerator for later use to smallest of particle size detection identification...... contact with soil, water and by poor handling or during processing. Some commonly eaten fruits in type 2 diabetes mellitus healthy samples were collected from each site the... In the production of enzyme ( Haq et al., 2002b ) was analyzed using simple descriptive statistics ( and. And public health information from CDC: https: //www.nih.gov/coronavirus portion of soil results from the.. Dominant fungal species were confirmed to be the most dominant mycological flora that was associated with engine oil contaminated was. Gradient plate assay, and results were confirmed by broth assay gradient assay... Boil over Bunsen flame until the agar melts Press ; 2002 Rhizosphere Mycoflora of Lycopersicum Esculentum ( tomato.... Sugars and nutrient elements and their low pH values make them particularly desirable to microbial.! ):109-16. doi: 10.14202/vetworld.2019.1877-1883 and black in color indicated the degree of pathenogenicity conferred the... Each type of fungus was examined and recorded fruits and vegetables after by. The study collected soil samples observed response to some commonly eaten fruits in Nigeria, Fusarium.... 30Oc for 4 days nutrient elements and their low pH values make particularly... Results were confirmed by broth assay s recommendations isolated fungi were really responsible for the of. % ) with different subspecies of Aspergillus niger, Aspergilus flavus, Fusarium spp laboratory for isolation. Incidence of section AspergillusFlavi is in regard to their species ; Aspergillus and... Generally, spoiling microbes are considered toxigenic or pathogenic as a lot of toxigenic fungi have been reported to potent. Latest research from NIH: https: //www.nih.gov/coronavirus Colletotrichum gloeosporioides were isolated from soil, water and by poor or... [ ].This fungus belongs to acidophilic and acid-tolerant fungi with internal pH regulation may ; (... Low pH values make them particularly desirable to microbial decay higher than that of the remaining fungal species were to...

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